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Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Name	Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26279
Category	Cell Line
Prices	$1,129.00
Sizes	1 vial
Applications	FC
Gene	TLR8
Species	Human
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR8 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor 8 (TLR8) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. TLR8 expression in this cell line has been tested by flow cytometry (fig. 5). Using the 96-well plate format assays, the TLR8 reporter cell line has been validated by DMSO tolerance (Fig. 1), cell number titration (Fig. 2), ligand dose response (Fig. 6), and cell stimulation time (Fig. 3). Contents: 3~4 x 10^6 cells Biosafety Level: 2
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Flow Cytometry: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - Intracellular staining of TLR8 in the TLR8 reporter cell line was analyzed by flow cytometry using a PE conjugated TLR8 antibody. Flow samples were prepared using the Intracellular TLR Staining Flow Kit. Purple: Cells without antibody; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 2 ug anti-TLR8-PE. Red: TLR8 reporter line stained with anti-TLR8-PE.

Ligand Activation: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - The TLR8 reporter line was plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of R848 in the presence of different amounts of DMSO as noted for 24 h. SEAP was then analyzed using SEAPorterTM Assay Kit.

Ligand Activation: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - The TLR8 reporter line was plated in 96-well plates at different concentrations of cells (100 ul/well) as noted in the figure. After 16 h, cells were stimulated with various amounts of R848, for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorterTM Assay Kit. Z' factor and Signal vs. Background (S/B) ratio were evaluated for each cell concentration set.

Ligand Activation: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - TLR8/NF-kB SEAPorter(TM) HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of R848 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: R848 activated the cell line in a dose response manner.

Ligand Activation: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - The TLR8 reporter line was plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of R848 for 24 h. SEAP was analyzed using SEAPorterTM Assay Kit. Dose-responsive percent activation of each sample well was calculated to yield the ligand EC50 value.

Ligand Activation: Human TLR8 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26279] - The TLR8 reporter line was plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of R848 for 6 h, 16 h or 24 h. SEAP was then analyzed using SEAPorterTM Assay Kit.